| 质粒名称: | pSG5 Large T |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 9053 |
| 存储实验室: | William Hahn |
|
载体骨架基本信息 |
|
| 载体名称: | pSG5 |
| 载体出品公司: | Stratagene |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4076 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | large T antigen,large T cDNA |
| 物种来源: | simian virus |
| 插入大小: | 2200 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | BamHI(未破坏) |
| 5' 测序引物 | T7 |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pSG5 Large T was a gift from William Hahn (Addgene plasmid # 9053 ; http://n2t.net/addgene:9053 ; RRID:Addgene_9053) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSG5 Large T 6252 bp ds-DNA circular SYN 17-MAY-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pSG5 Large T
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6252)
TITLE T antigen
REFERENCE 2 (bases 1 to 6252)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 17, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6252
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(32..52)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 140..350
/label=SV40 promoter
/note="SV40 early promoter"
promoter 162..358
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 209..344
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 271..290
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 389..961
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind 969..988
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
primer_bind 1016..1035
/label=T7
/note="T7 promoter, forward primer"
promoter 1016..1034
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1076..3202
/codon_start=1
/product="large T antigen from SV40 (simian virus 40)"
/label=large T antigen
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
CFKKPPTPPPEPET"
polyA_signal 3253..3374
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3290..3309)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3344..3363
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(3408..3425)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3579..4167)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3659..3678)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4338..5198)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4961..4980
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5199..5303)
/gene="bla"
/label=AmpR promoter
primer_bind 5371..5389
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(5427..5449)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 5549..5568
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin complement(5635..6090)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5772..5793)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 5984..6003
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6216..6238
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6230..6247
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6231..6247
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gtcgacttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaagg
121 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
181 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
241 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
301 ctctgagcta ttccagaagt agtgaagagg cttttttgga ggcctaggct tttgcaaaaa
361 gctccggatc gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt
421 tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag
481 aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt
541 tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc
601 gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga
661 ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac
721 ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca
781 tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca tcctggtcat
841 catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata
901 ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct ttttcctaca
961 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgtaata
1021 cgactcacta tagggcgaat tcggatcccg cgaccggtcg accagctttg caaagatgga
1081 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
1141 tgcctggggg aatattcctc tgatgagaaa ggcatattta aaaaaatgca aggagtttca
1201 tcctgataaa ggaggagatg aagaaaaaat gaagaaaatg aatactctgt acaagaaaat
1261 ggaagatgga gtaaaatatg ctcatcaacc tgactttgga ggcttctggg atgcaactga
1321 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
1381 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
1441 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
1501 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
1561 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
1621 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
1681 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
1741 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atccattttc
1801 tgttattgag gaaagtttgc caggtgggtt aaaggagcat gattttaatc cagaagaagc
1861 agaggaaact aaacaagtgt cctggaagct tgtaacagag tatgcaatgg aaacaaaatg
1921 tgatgatgtg ttgttattgc ttgggatgta cttggaattt cagtacagtt ttgaaatgtg
1981 tttaaaatgt attaaaaaag aacagcccag ccactataag taccatgaaa agcattatgc
2041 aaatgctgct atatttgctg acagcaaaaa ccaaaaaacc atatgccaac aggctgttga
2101 tactgtttta gctaaaaagc gggttgatag cctacaatta actagagaac aaatgttaac
2161 aaacagattt aatgatcttt tggataggat ggatataatg tttggttcta caggctctgc
2221 tgacatagaa gaatggatgg ctggagttgc ttggctacac tgtttgttgc ccaaaatgga
2281 ttcagtggtg tatgactttt taaaatgcat ggtgtacaac attcctaaaa aaagatactg
2341 gctgtttaaa ggaccaattg atagtggtaa aactacatta gcagctgctt tgcttgaatt
2401 atgtgggggg aaagctttaa atgttaattt gcccttggac aggctgaact ttgagctagg
2461 agtagctatt gaccagtttt tagtagtttt tgaggatgta aagggcactg gaggggagtc
2521 cagagatttg ccttcaggtc agggaattaa taacctggac aatttaaggg attatttgga
2581 tggcagtgtt aaggtaaact tagaaaagaa acacctaaat aaaagaactc aaatatttcc
2641 ccctggaata gtcaccatga atgagtacag tgtgcctaaa acactgcagg ccagatttgt
2701 aaaacaaata gattttaggc ccaaagatta tttaaagcat tgcctggaac gcagtgagtt
2761 tttgttagaa aagagaataa ttcaaagtgg cattgctttg cttcttatgt taatttggta
2821 cagacctgtg gctgagtttg ctcaaagtat tcagagcaga attgtggagt ggaaagagag
2881 attggacaaa gagtttagtt tgtcagtgta tcaaaaaatg aagtttaatg tggctatggg
2941 aattggagtt ttagattggc taagaaacag tgatgatgat gatgaagaca gccaggaaaa
3001 tgctgataaa aatgaagatg gtggggagaa gaacatggaa gactcagggc atgaaacagg
3061 cattgattca cagtcccaag gctcatttca ggcccctcag tcctcacagt ctgttcatga
3121 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
3181 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacggg gatccagatc
3241 ttattaaagc agaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
3301 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
3361 atcaatgtat cttatcatgt ctggtcgact ctagactctt ccgcttcctc gctcactgac
3421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
3481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
3541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
3601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
3661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
3721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
3781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
3841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
3901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
3961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
4021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
4081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
4141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
4201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
4261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
4321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
4381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
4441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
4501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
4561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
4621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
4681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
4741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
4801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
4861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
4921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
4981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
5041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
5101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
5161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
5221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
5281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
5341 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
5401 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
5461 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
5521 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
5581 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg
5641 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
5701 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
5761 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
5821 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
5881 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
5941 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
6001 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
6061 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg
6121 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
6181 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
6241 ggccagtgaa tt
//
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